Expertise & Services

Our core facility specializes in the generation, processing, statistical analysis, integration, and visualization of high-density genomics and proteomics data for translational research. 

Laboratory expertise includes:

  • Experimental design
  • Sample preparation (e.g. DNA/RNA extraction and quality control)
  • Library preparation and enrichment for bulk DNA and RNA sequencing (does not differentiate among cell types within the sample)
  • Single-cell RNA sequencing
  • Protein quantification by liquid chromatography-mass spectrometry
  • Analytical pharmacology
  • Pilot projects to evaluate technology performance
  • Training and use of instruments

Data analysis expertise includes tailored bioinformatics support:

  • Use of discrete algorithms
  • Statistical analysis
  • The computational workflows to perform single-omic and integrated analyses of genomic, transcriptomic and proteomic data.
  • High-throughput data analysis is done on a high-performance cluster system.

Supported applications

  • 10x Genomics single-cell sequencing
  • Exome sequencing for integrative analysis of SNVs and CNVs
  • mRNA sequencing for gene expression or whole transcriptome analysis
  • Targeted DNA and RNA sequencing
  • Small RNA sequencing
  • NGS library sequencing
  • Qualitative (label-free) and quantitative (TMT, SILAC) differential analysis of protein levels
  • Analytical chemistry and clinical pharmacology

Major equipment

  • Maxwell RSC for automated DNA and RNA extraction system
  • Agilent TapeStation 4200 for quality control of DNA and RNA
  • Covaris M220 focused-ultrasonicator for sample preparation
  • ThermoFisher Zephyr NGS liquid handler systems for automated preparation of NGS sequencing libraries
  • Illumina NextSeq, MiSeq, MiniSeq
  • nanoLC Orbitrap Lumos, standard LC triple quad, standard LC with photometric detection for LC-MSMS
  • Computing infrastructure: 28 nodes / 896 cores / 2Pb of storage

 

Updated November 27, 2020

A World Class Centre

The Vancouver Prostate Centre (VPC) has a track record of success that has earned it a reputation as one of the world’s most respected cancer facilities. It is a National Centre of Excellence and a designated Centre of Excellence for Commercialization and Research.

Events

VPC Terry Fox team 2016

Terry Fox Run - September 20, 2020

The 40th annual Terry Fox Run takes place on Sunday September 20 across Canada, as a virtual event.  Please consider donating to or joining our TFRI-VPC team as we raise funds to enable The Terry Fox Foundation & The Terry Fox Research Institute to continue their support of innovative and progressive cancer research programs

Employment

Work at the Vancouver Prostate Centre

Thursday, March 4, 2021

Dr. Collins awarded CIHR Project Grant

Dr. Colin Collins
Friday, January 22, 2021

Dr. Nappi receives MSFHR 2020 Health Professional-Investigator Award