Expertise & Services

Our core facility specializes in the generation, processing, statistical analysis, integration, and visualization of high-density genomics and proteomics data for translational research. 

Laboratory expertise includes:

  • Experimental design
  • Sample preparation (e.g. DNA/RNA extraction and quality control)
  • Library preparation and enrichment for bulk DNA and RNA sequencing (does not differentiate among cell types within the sample)
  • Single-cell RNA sequencing
  • Protein quantification by liquid chromatography-mass spectrometry
  • Analytical pharmacology
  • Pilot projects to evaluate technology performance
  • Training and use of instruments

Data analysis expertise includes tailored bioinformatics support:

  • Use of discrete algorithms
  • Statistical analysis
  • The computational workflows to perform single-omic and integrated analyses of genomic, transcriptomic and proteomic data.
  • High-throughput data analysis is done on a high-performance cluster system.

Supported applications

  • 10x Genomics single-cell sequencing
  • Exome sequencing for integrative analysis of SNVs and CNVs
  • mRNA sequencing for gene expression or whole transcriptome analysis
  • Targeted DNA and RNA sequencing
  • Small RNA sequencing
  • NGS library sequencing
  • Qualitative (label-free) and quantitative (TMT, SILAC) differential analysis of protein levels
  • Analytical chemistry and clinical pharmacology

Major equipment

  • Maxwell RSC for automated DNA and RNA extraction system
  • Agilent TapeStation 4200 for quality control of DNA and RNA
  • Covaris M220 focused-ultrasonicator for sample preparation
  • ThermoFisher Zephyr NGS liquid handler systems for automated preparation of NGS sequencing libraries
  • Illumina NextSeq, MiSeq, MiniSeq
  • nanoLC Orbitrap Lumos, standard LC triple quad, standard LC with photometric detection for LC-MSMS
  • Computing infrastructure: 28 nodes / 896 cores / 2Pb of storage

 

Updated November 27, 2020

A World Class Centre

The Vancouver Prostate Centre (VPC) has a track record of success that has earned it a reputation as one of the world’s most respected cancer facilities. It is a National Centre of Excellence and a designated Centre of Excellence for Commercialization and Research.

Events

Conference Flyer Page 1

Breast & Prostate Cancer half-day virtual conference: Saturday, September 18th, 2021

The What you Need to Know for Your Patients Post Breast & Prostate Cancer Conference, taking place online on Saturday, September 18th, 2021 from 8:00am – 1:30pm, is an accredited virtual half-day conference focusing on screening, treatment, side-effect management & emerging new therapies for breast & prostate cancer patients. Click here for more information and to register. 

Dr. Peter Black, team captain

Terry Fox Run fundraiser: September 19th, 2021

The 41st Annual Terry Fox Run is taking place on Sunday September 19, 2021. If you are able, please consider donating to our team (tax receipts issued), raising funds for the Terry Fox Foundation's ground-breaking cancer research. 

Employment

Work at the Vancouver Prostate Centre

Wednesday, September 8, 2021

Recent publication proposes a potential new therapeutic approach to treatment-resistant prostate cancer

Tuesday, August 3, 2021

Dr. Cherkasov receives 2021 Faculty of Medicine Distinguished Achievement Award

Dr. Cherkasov