Genomic Analysis
A translational genomics platform for cross-disciplinary research and innovation
The Genomics Core facility at the Vancouver Prostate Centre specializes in the acquisition, processing, statistical analysis, integration, and visualization of next-generation sequencing and mass spectrometry data to enable and advance basic and translational research.
Our mission is to provide researchers and healthcare professionals with the best scientific support and high-quality multi-omics data. By combining cutting-edge technology with expert knowledge and analysis, we aim to advance our understanding of diseases to help develop new and innovative treatments.
We offer essential recommendations for generating and analyzing complex data from standard and challenging clinical samples to support the needs of academic and clinical scientists from not-for-profit research institutions and biotechnology companies. We emphasize collaborations to improve and grow our expertise continually. We help manage research projects from experimental design to data interpretation and support the researchers’ grant applications and publications.
We offer economically priced services based on a cost-recovery, not-for-profit model. Additionally, we provide training and educational opportunities to help researchers stay updated with the latest advances in the field.
Between 2012 and 2024, our genomics, proteomics and analytical expertise have been acknowledged in 50 publications.
Our core facility offers:
Collaborative Teamwork
- Capitalize on analytical methods without becoming an expert.
- Extensive experience and highly trained personnel.
- Experimental design and method development.
- Sample preparation (tissue processing, DNA/RNA extraction and QC).
- High-quality results for publications and grant proposals.
- Cost-recovery, not-for-profit model, economical and personalized solutions.
- Personalized solutions to address each project.
- Pilot projects to evaluate technology performance.
- Emphasize collaboration.
Genomics applications
- 10x Genomics and Fluent Biosciences single-cell sequencing.
- Whole genome and exome sequencing.
- RNA sequencing for gene expression.
- Targeted DNA and RNA sequencing
- Small RNA sequencing
- Library sequencing
Mass Spectrometry Applications
- Experimental design and method development.
- Sample preparation.
- IP, IGD, ISD.
- Qualitative (label-free) and quantitative (TMT, SILAC).
- Analytical chemistry and clinical pharmacology.
Data analysis expertise includes tailored bioinformatics support:
- Use of discrete algorithms
- Statistical analysis
- Computational workflows to perform single-omic and integrated genomic, transcriptomic, and proteomic data analyses.
- High-throughput data analysis is done on a high-performance cluster system.
Major equipment
- Precellys Evolution for homogenization applications.
- Maxwell RSC system for DNA and RNA extraction.
- Agilent TapeStation 4200 for the quality control of DNA and RNA.
- Covaris focused-ultrasonicator for DNA shearing.
- 10x Genomics Chromium for the preparation of single-cell RNA and DNA libraries.
- Complete Genomics DNBSEQ-G400RS sequencer. Data output/run up to 3.6 G reads for 1.4 Tb data; max read length: SE400 & PE200. Capacity up to 48 WES at 150x in 3 days
- Complete Genomics DNBSEQ-T7 sequencer. Data output/run up to 23 G reads for 6 Tb data; max read length: PE150. Capacity up to 48 human WGS at 30x in 30 hrs.
- Thermo Fisher nanoLC Orbitrap Lumos, standard LC triple quad, standard LC with photometric detection for LC-MSMS
- Computing infrastructure: 28 nodes / 896 cores / 2Pb of storage.
Updated: August 22, 2024
For more information about how LAGA's technology can benefit your project, please contact:
Dr. Stephane LeBihan, Ph.D.
Manager, Genomics Core
Tel: 778-991-4506
Email: Click here to contact Dr. LeBihan